![](https://parts.igem.org/images/partbypart/icon_reporter.png)
Reporter
Part:BBa_K3281009:Design
Designed by: Patrick Dickinson Group: iGEM19_Harvard (2019-10-21)
tTA (TANGO assay) Reporter - eGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The actin-localizing portion of eGFP was removed in cloning as we had no particular reason to fluoresce just actin, and the resulting reduction in amplicon length made for more efficient Gibson assembly.
Source
tTA sequence was derived from an addgene plasmid, and the source DNA can be found at this address:
https://www.addgene.org/browse/sequence/204193/
eGFP was cloned from an eGFP-actin localizing reporter that was in stock at Harvard University's Silver Lab.